E-ISSN 2231-170X | ISSN 2231-1696
 

Original Article 


Neurodegenerative changes in different regions of brain, spinal cord and sciatic nerve of rats treated with sodium fluoride

P. Yugandhar Reddy, K. Pratap Reddy, K. Praveen Kumar.

Abstract
Fluoride is known to cross the blood-brain barrier and alter the structure and function of neural tissue. There are few authoritative reports on neurodegenerative changes in hippocampus, neocortex, cerebellum, spinal cord and sciatic nerve in fluoride intoxication. We report the alterations in the structure of neuronal tissue after chronic administration of sodium fluoride (for 60days) to rats. Twelve male Wistar rats were divided equally into two groups: one group received 20 ppm of sodium fluoride (NaF) and the other group (which served as a control) received tap water for 60days.
The body weights and organic somatic index of brain in the sodium fluoride treated animals were significantly reduced, relative to the control group. Tissue fluoride levels of hippocampus, neocortex, cerebellum, spinal cord and sciatic nerve, all increased significantly in fluoride treated rats. Electron microscopy of the hippocampus, neocortex, cerebellum, spinal cord and sciatic nerve showed neurodegenerative changes in the NaF treated group compared to controls. Axon deterioration, myelin sheath degeneration and dark cells with scanty cytoplasm were observed in spinal cord and sciatic nerve in the treated group. Other distinctive morphological alterations observed were: vacuolated swollen mitochondria in neocortex, hippocampus and cerebellum; myelinated fibers with breaks in continuity (axon partly preserved and partly vacuolated) in hippocampus; myelin splitting and vacuolated schwann cell within the cerebellum and sciatic nerve respectively. Thus, neurodegeneration was clearly evident in the hippocampus, neocortex, cerebellum, spinal cord and sciatic nerve on fluoride exposure.

Key words: Sciatic nerve, Cerebellum, Sodium fluoride, Hippocampus, Transmission electron microscope


 
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REFERENCES
1. Waldbott GL, Burgstahler AW, McKinney HL. Fluoride in soft tissues In: Fluoridation: the great dilemma. Coronado Press., Lawrence, Kansas, pp. 160-161, 1978.
2. Sharma A, Chinoy NJ. Role of free radicals in fluoride-induced toxicity in liver and kidney of mice and its reversal. Fluoride 1998; 31: S26.
3. Liu WX. Experimental study of behavior and cerebral morphology of rat pups generated by fluorotic female rat. Zhonghua Bing Li Xuc ZaZhi 1989; 4: 290-292.
4. Zhan XU, Wang L. Effect of fluorosis on lipid peroxidation and antioxidant system in young pigs. Fluoride 2005; 38(2): 157-161.
5. Zhan XA, Wang M, Xu ZR, Li JX. Toxic effects of fluoride on kidney function and histological structure in young pigs. Fluoride 2006; 39(1): 22-26.
6. Shan Guan CM. The non skeletal lesions of endemic fluorosis. Chin J Intern Med 1982; 21:217-219.
7. Ding LI. The nervous system complications of chronic fluorosis. Chin Endem 1983; 2: 97-98.
8. Mullenix PJ, Denbesten PK, Schuniora K, Kernand WJ. Neurotoxicity of sodium fluoride in rats. Neurotoxicol Teratol 1995; 17(2): 169-177. [DOI via Crossref]   
9. Varner JA, Hovarth W, Jensen KF, Isaacson RL. Chronic administration of aluminium fluoride or sodium fluoride to rats in drinking water: alteration in cerebrovascular integrity, Brain Res 1998; 784(1-2): 284-298. [DOI via Crossref]   
10. Shivarajshankara YM. Histological changes in the brain of young, fluoride intoxication rats. Fluoride 2002; 35(1):12-21.
11. Cepák V, Komárek J. Cytomorphology of six halotolerant coccoid cyanobacteria using DAPI fluorescent and transmission electron microscopy, compared with molecular data Fottea 2010; 10(2): 229–234. [DOI via Crossref]   
12. Singh A, Jolly SS. Endemic fluorosis with particular reference to fluorotic radioculo-myelopathy. Quart J med 1961; 30: 357-372. [Pubmed]   
13. Burgstahler AW, Colquhoun JC. Neurotoxicity of fluoride. Fluoride 1996; 29: 57-58.
14. Vani ML and Reddy KP. Effects of fluoride accumulation on some enzymes of brain and gastrocnemius muscle of mice. Fluoride 2000; 33: 17-26.
15. Zhang Z, XU X, Shen X, XU X. Effect of fluoride exposure on synaptic structure of brain area related to learning memory in mice. Wei Sheng Yan Jiu 1999; 28(4): 210-212. [Pubmed]   
16. Varner JA, Hule CW, Hovarth W, Jensen KF, Isaacson RL. Chronic aluminium fluoride administration: II selected Histological observation. Neuro Science Res Comm 1993; 13: 99-104.
17. Isokawa M, Levesque MF. Increased NMDA responses and dentritic degeneration in human epileptic hippocampal neurons in slices. Neuro Sci Lett 1991; 132: 212-216. [DOI via Crossref]   
18. Chirumari K and Reddy PK. Dose-dependent effects of fluoride on neurochemical milieu in the hippocampus and neocortex of rat brain. Fluoride 2007; 40(2): 101-110
19. Shashi A, Sing JP, Thapar SP. Effect of long term administration of fluoride on the levels of protein, free amino acids and RNA in rabbit brain. Fluoride 1994; 27:155-159.
20. Pellegrini- Giampietro DE, Cherici G, Alesiani M, Carla V, Moroni F. Excitatory amino acid release from rat hippocampal slices as a consequence of free-radical formation. J Neuro Chem 1988; 51: 1960-1963. [DOI via Crossref]   
21. Blaylock RL. Excitotoxicity: a possible central mechanism in fluoride neurotoxicity. Fluoride 2004; 37: 301-314.
22. Blanc EM, Keller JN, Fernandez S, Mattson MP. 4-Hydroxynonenol, a lipid peroxidation product impairs glutamate- transport in cortical astrocytes. Glia 1998; 22: 149-160. [DOI via Crossref]   
23. Xu S, Shu B, Chen Z. Effect of fluoride on activities of nitric oxide synthase in rat brain (Abstract). Fluoride 2001; 34: 84.
24. Cassina R, Radi R. Differential inhibitory action of nitric oxide and peroxinitrite on mitochondrial electron transport. Arch Biochem Biophys 1997; 328: 309-316. [DOI via Crossref]    [Pubmed]   
25. Bolanos JP, Almeida A, Stewart V, Peuchen S, Land JM, Clark JB. Nitric oxide mediated mitochondrial damage in the brain; mechanism and implications for neurodegenerative diseases. J Neurochem 1997; 68: 2227-2240. [DOI via Crossref]    [Pubmed]   

How to Cite this Article
Pubmed Style

P. Yugandhar Reddy, K. Pratap Reddy, K. Praveen Kumar. Neurodegenerative changes in different regions of brain, spinal cord and sciatic nerve of rats treated with sodium fluoride. J Med Allied Sci. 2011; 1(1): 30-35.


Web Style

P. Yugandhar Reddy, K. Pratap Reddy, K. Praveen Kumar. Neurodegenerative changes in different regions of brain, spinal cord and sciatic nerve of rats treated with sodium fluoride. https://jmas.in/?mno=210079 [Access: January 12, 2023].


AMA (American Medical Association) Style

P. Yugandhar Reddy, K. Pratap Reddy, K. Praveen Kumar. Neurodegenerative changes in different regions of brain, spinal cord and sciatic nerve of rats treated with sodium fluoride. J Med Allied Sci. 2011; 1(1): 30-35.



Vancouver/ICMJE Style

P. Yugandhar Reddy, K. Pratap Reddy, K. Praveen Kumar. Neurodegenerative changes in different regions of brain, spinal cord and sciatic nerve of rats treated with sodium fluoride. J Med Allied Sci. (2011), [cited January 12, 2023]; 1(1): 30-35.



Harvard Style

P. Yugandhar Reddy, K. Pratap Reddy, K. Praveen Kumar (2011) Neurodegenerative changes in different regions of brain, spinal cord and sciatic nerve of rats treated with sodium fluoride. J Med Allied Sci, 1 (1), 30-35.



Turabian Style

P. Yugandhar Reddy, K. Pratap Reddy, K. Praveen Kumar. 2011. Neurodegenerative changes in different regions of brain, spinal cord and sciatic nerve of rats treated with sodium fluoride. Journal of Medical and Allied Sciences, 1 (1), 30-35.



Chicago Style

P. Yugandhar Reddy, K. Pratap Reddy, K. Praveen Kumar. "Neurodegenerative changes in different regions of brain, spinal cord and sciatic nerve of rats treated with sodium fluoride." Journal of Medical and Allied Sciences 1 (2011), 30-35.



MLA (The Modern Language Association) Style

P. Yugandhar Reddy, K. Pratap Reddy, K. Praveen Kumar. "Neurodegenerative changes in different regions of brain, spinal cord and sciatic nerve of rats treated with sodium fluoride." Journal of Medical and Allied Sciences 1.1 (2011), 30-35. Print.



APA (American Psychological Association) Style

P. Yugandhar Reddy, K. Pratap Reddy, K. Praveen Kumar (2011) Neurodegenerative changes in different regions of brain, spinal cord and sciatic nerve of rats treated with sodium fluoride. Journal of Medical and Allied Sciences, 1 (1), 30-35.