E-ISSN 2231-170X | ISSN 2231-1696
 

Original Article
Online Published: 11 Dec 2015
 


Genetic diversity among Puntius sophore complex using restriction fragment length polymorphism

Smitha Balaraj, Madompoyil Basheer.


Abstract
Restriction fragment length polymorphism (RFLP) analysis of ge-nomic DNA was employed to characterize species of Puntius sophore complex, a benthopelagic freshwater fish species. Samples of five species of Puntius viz, Puntius sophore, Puntius amphibius, Puntius chola, Puntius bimaculatus and Puntius dorsalis from the East-flowing Tamiraparani and Kuzhithurai and West-flowing Tamiraparani river basins (Western Ghats) were analyzed. The restriction enzyme employed (Hind III) generated species-specific DNA patterns for each of the five species. DNA exhibited considerable polymorphism among species. All samples showed relatively high values of diversity. The relation between species is discussed.

Key words: RFLP, Hind III, Genetic diversity, Puntius sophore complex


 
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Pubmed Style

Smitha Balaraj, Madompoyil Basheer. Genetic diversity among Puntius sophore complex using restriction fragment length polymorphism. J Med Allied Sci. 2012; 2(2): 49-53.


Web Style

Smitha Balaraj, Madompoyil Basheer. Genetic diversity among Puntius sophore complex using restriction fragment length polymorphism. https://jmas.in/?mno=210562 [Access: September 12, 2024].


AMA (American Medical Association) Style

Smitha Balaraj, Madompoyil Basheer. Genetic diversity among Puntius sophore complex using restriction fragment length polymorphism. J Med Allied Sci. 2012; 2(2): 49-53.



Vancouver/ICMJE Style

Smitha Balaraj, Madompoyil Basheer. Genetic diversity among Puntius sophore complex using restriction fragment length polymorphism. J Med Allied Sci. (2012), [cited September 12, 2024]; 2(2): 49-53.



Harvard Style

Smitha Balaraj, Madompoyil Basheer (2012) Genetic diversity among Puntius sophore complex using restriction fragment length polymorphism. J Med Allied Sci, 2 (2), 49-53.



Turabian Style

Smitha Balaraj, Madompoyil Basheer. 2012. Genetic diversity among Puntius sophore complex using restriction fragment length polymorphism. Journal of Medical and Allied Sciences, 2 (2), 49-53.



Chicago Style

Smitha Balaraj, Madompoyil Basheer. "Genetic diversity among Puntius sophore complex using restriction fragment length polymorphism." Journal of Medical and Allied Sciences 2 (2012), 49-53.



MLA (The Modern Language Association) Style

Smitha Balaraj, Madompoyil Basheer. "Genetic diversity among Puntius sophore complex using restriction fragment length polymorphism." Journal of Medical and Allied Sciences 2.2 (2012), 49-53. Print.



APA (American Psychological Association) Style

Smitha Balaraj, Madompoyil Basheer (2012) Genetic diversity among Puntius sophore complex using restriction fragment length polymorphism. Journal of Medical and Allied Sciences, 2 (2), 49-53.