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Original Article
Online Published: 13 Dec 2015
 


Method development and validation of paracetamol drug by RP-HPLC

T. A. Phazna Devi, Aravind Setti, S. Srikanth, Sivaramaiah Nallapeta, Smita C. Pawar, J. Venkateshwara Rao.


Abstract
A simple and reproducible method was developed for paracetamol by Reverse Phase High Performance Liquid Chromatography (RP-HPLC). Paracetamol was separated on C18 column [4.6x250mm, particle size 5μm], using ortho phosphoric acid buffer with pH of 3.5 at the UV detection of 207nm. Isocratic elution of acetonitrile (ACN) and water was used as a mobile phase with various ratios and flow rates, eventually 25:75 v/v ACN and water was being set with the flow rate of 1mL/min. The statistical validation parameters such as linearity, accuracy, precision, inter-day and intra-day variation were checked, further the limit of detection and limit of quantification of paracetamol concentrations were found to be 120ng/mL and 360ng/mL. Recovery and assay studies of paracetamol were within 99 to 102% indicating that the proposed method can be adoptable for quality control analysis of paracetamol.

Key words: RP-HPLC, Validation, Paracetamol, Acetonitrile


 
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How to Cite this Article
Pubmed Style

T. A. Phazna Devi, Aravind Setti, S. Srikanth, Sivaramaiah Nallapeta, Smita C. Pawar, J. Venkateshwara Rao. Method development and validation of paracetamol drug by RP-HPLC. J Med Allied Sci. 2013; 3(1): 8-14.

Web Style

T. A. Phazna Devi, Aravind Setti, S. Srikanth, Sivaramaiah Nallapeta, Smita C. Pawar, J. Venkateshwara Rao. Method development and validation of paracetamol drug by RP-HPLC. https://jmas.in/?mno=210684 [Access: December 31, 2024].

AMA (American Medical Association) Style

T. A. Phazna Devi, Aravind Setti, S. Srikanth, Sivaramaiah Nallapeta, Smita C. Pawar, J. Venkateshwara Rao. Method development and validation of paracetamol drug by RP-HPLC. J Med Allied Sci. 2013; 3(1): 8-14.


Vancouver/ICMJE Style

T. A. Phazna Devi, Aravind Setti, S. Srikanth, Sivaramaiah Nallapeta, Smita C. Pawar, J. Venkateshwara Rao. Method development and validation of paracetamol drug by RP-HPLC. J Med Allied Sci. (2013), [cited December 31, 2024]; 3(1): 8-14.


Harvard Style

T. A. Phazna Devi, Aravind Setti, S. Srikanth, Sivaramaiah Nallapeta, Smita C. Pawar, J. Venkateshwara Rao (2013) Method development and validation of paracetamol drug by RP-HPLC. J Med Allied Sci, 3 (1), 8-14.


Turabian Style

T. A. Phazna Devi, Aravind Setti, S. Srikanth, Sivaramaiah Nallapeta, Smita C. Pawar, J. Venkateshwara Rao. 2013. Method development and validation of paracetamol drug by RP-HPLC. Journal of Medical and Allied Sciences, 3 (1), 8-14.


Chicago Style

T. A. Phazna Devi, Aravind Setti, S. Srikanth, Sivaramaiah Nallapeta, Smita C. Pawar, J. Venkateshwara Rao. "Method development and validation of paracetamol drug by RP-HPLC." Journal of Medical and Allied Sciences 3 (2013), 8-14.


MLA (The Modern Language Association) Style

T. A. Phazna Devi, Aravind Setti, S. Srikanth, Sivaramaiah Nallapeta, Smita C. Pawar, J. Venkateshwara Rao. "Method development and validation of paracetamol drug by RP-HPLC." Journal of Medical and Allied Sciences 3.1 (2013), 8-14. Print.


APA (American Psychological Association) Style

T. A. Phazna Devi, Aravind Setti, S. Srikanth, Sivaramaiah Nallapeta, Smita C. Pawar, J. Venkateshwara Rao (2013) Method development and validation of paracetamol drug by RP-HPLC. Journal of Medical and Allied Sciences, 3 (1), 8-14.


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