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Original Article
Online Published: 17 Dec 2015
 


Comparison of conventional diagnostic modalities, BACTEC culture with polymerase chain reaction for diagnosis of extra-pulmonary tuberculosis

Mohammed Abdul Mohi Siddiqui, P. R. Anuradha, K. Nagamani, P. H. Vishnu.


Abstract
This study attempts to determine and compare the sensitivity, specificity and turnaround time of conventional diagnostic modalities, BACTEC culture and polymerase chain reaction test for diagnosis of extra-pulmonary tuberculosis. One hundred clinically suspected patients of extra-pulmonary tuberculosis were studied. Specimens from these patients were processed for Ziehl-Neelsen (ZN) stain and culture for M. tuberculosis was done with inoculation on Lowenstein-Jensen (LJ) medium as well as the BACTEC MGIT 960 TB system. All the samples were processed for PCR amplification with primers targeting 123 bp fragment of insertion element IS6110 sequence of M. tuberculosis complex. Out of the hundred extra-pulmonary samples processed, 5% were positive by ZN staining, 15% were positive by both LJ culture and BACTEC MGIT 960 TB culture and 70% were positive by PCR. Those samples positive by ZN smear, LJ culture and BACTEC culture were all found to be 100% positive by PCR. The mean detection time for M. tuberculosis was 23.13 days by LJ Medium culture, 9.86 days by BACTEC MGIT 960 TB culture and less than one day by PCR. PCR as a diagnostic tool is more sensitive and useful in diagnosis of extra-pulmonary form of tuberculosis when compared to conventional methods or BACTEC culture by definitely shortening the time with early initiation of anti-tubercular treatment and can prevent disease progressing towards irreversible tissue damage.

Key words: BACTEC MGIT 960 TB system, Extra-pulmonary tuberculosis, IS6110 sequence, LJ Medium, PCR, ZN staining


 
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How to Cite this Article
Pubmed Style

Mohammed Abdul Mohi Siddiqui, P. R. Anuradha, K. Nagamani, P. H. Vishnu. Comparison of conventional diagnostic modalities, BACTEC culture with polymerase chain reaction for diagnosis of extra-pulmonary tuberculosis. J Med Allied Sci. 2013; 3(2): 53-58.

Web Style

Mohammed Abdul Mohi Siddiqui, P. R. Anuradha, K. Nagamani, P. H. Vishnu. Comparison of conventional diagnostic modalities, BACTEC culture with polymerase chain reaction for diagnosis of extra-pulmonary tuberculosis. https://jmas.in/?mno=211115 [Access: September 12, 2024].

AMA (American Medical Association) Style

Mohammed Abdul Mohi Siddiqui, P. R. Anuradha, K. Nagamani, P. H. Vishnu. Comparison of conventional diagnostic modalities, BACTEC culture with polymerase chain reaction for diagnosis of extra-pulmonary tuberculosis. J Med Allied Sci. 2013; 3(2): 53-58.


Vancouver/ICMJE Style

Mohammed Abdul Mohi Siddiqui, P. R. Anuradha, K. Nagamani, P. H. Vishnu. Comparison of conventional diagnostic modalities, BACTEC culture with polymerase chain reaction for diagnosis of extra-pulmonary tuberculosis. J Med Allied Sci. (2013), [cited September 12, 2024]; 3(2): 53-58.


Harvard Style

Mohammed Abdul Mohi Siddiqui, P. R. Anuradha, K. Nagamani, P. H. Vishnu (2013) Comparison of conventional diagnostic modalities, BACTEC culture with polymerase chain reaction for diagnosis of extra-pulmonary tuberculosis. J Med Allied Sci, 3 (2), 53-58.


Turabian Style

Mohammed Abdul Mohi Siddiqui, P. R. Anuradha, K. Nagamani, P. H. Vishnu. 2013. Comparison of conventional diagnostic modalities, BACTEC culture with polymerase chain reaction for diagnosis of extra-pulmonary tuberculosis. Journal of Medical and Allied Sciences, 3 (2), 53-58.


Chicago Style

Mohammed Abdul Mohi Siddiqui, P. R. Anuradha, K. Nagamani, P. H. Vishnu. "Comparison of conventional diagnostic modalities, BACTEC culture with polymerase chain reaction for diagnosis of extra-pulmonary tuberculosis." Journal of Medical and Allied Sciences 3 (2013), 53-58.


MLA (The Modern Language Association) Style

Mohammed Abdul Mohi Siddiqui, P. R. Anuradha, K. Nagamani, P. H. Vishnu. "Comparison of conventional diagnostic modalities, BACTEC culture with polymerase chain reaction for diagnosis of extra-pulmonary tuberculosis." Journal of Medical and Allied Sciences 3.2 (2013), 53-58. Print.


APA (American Psychological Association) Style

Mohammed Abdul Mohi Siddiqui, P. R. Anuradha, K. Nagamani, P. H. Vishnu (2013) Comparison of conventional diagnostic modalities, BACTEC culture with polymerase chain reaction for diagnosis of extra-pulmonary tuberculosis. Journal of Medical and Allied Sciences, 3 (2), 53-58.


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