E-ISSN 2231-170X | ISSN 2231-1696
 

Original Article
Online Published: 12 Sep 2023
 


Rationale of using SARS-CoV-2 Rapid Antigen Test as a Point of care device in resource poor clinical set up: A comparative study in a peripheral health set up to meet up pandemic needs

Protik Mondal, Purba Mukherjee, Debasis Das, Tapan Kumar Mandal, Piyali Haldar, Shiv Sekhar Chatterjee, Utpal Dan, Ramaprasasd Roy.


Abstract
COVID-19 testing with RT-PCR may result in late detection of disease. Rapid Antigen Detection Test is much faster, easy to perform and suitable for population wise testing strategies. Our study assesses the diagnostic accuracy of Rapid Antigen Test (RAT) compared to RT-PCR in peripheral health care set up. The study was aimed to detect the diagnostic accuracy of the COVID-19 antigen test such as to understand its position as a point of care device in a resource poor clinical set up during a rapidly spreading and high mortality ridden pandemic. In this study we compared SARS-CoV2 rapid antigen test and RT-PCR by considering that RT-PCR is the gold standard test in a rapidly arranged molecular diagnostic lab. We analyzed total 389 samples who have undergone both rapid antigen test and RT-PCR. The correlation between Ct values in RT-PCR test and RAT positivity was analyzed. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy were found to be 75.47% (61.72-86.24%), 92.26% (88.87-94.88%), 33.92% (25.60-43.36%), 98.62% (97.8-99.14%) and 91.42% (88.19-94.19%), respectively. The k-coefficient of agreement between RAT and RTPCR was 0.615 ± 0.056 (0.504-0.724). For specimens with Ct value < 20, 20-25, 26-30, 30-35 and >35 were 100%, 90.3%, 80.3%, 60% for Ct value and 25% respectively. Our results suggest that RAT may be considered as an alternative to RT-PCR testing in peripheral health care set up. We reveal the importance of implementing rapid antigen test properly combined with clinical assessment of symptomatic patients so that it can be used in primary health care where RT-PCR testing facility is not available.

Key words: COVID-19, Ct value, Point of Care diagnostic test, Rapid Antigen Test, RT-PCR, SARS-CoV-2


 
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How to Cite this Article
Pubmed Style

Mondal P, Mukherjee P, Das D, Mandal TK, Haldar P, Chatterjee SS, Dan U, Roy R. Rationale of using SARS-CoV-2 Rapid Antigen Test as a Point of care device in resource poor clinical set up: A comparative study in a peripheral health set up to meet up pandemic needs. J Med Allied Sci. 2022; 12(2): 47-51. doi:10.5455/jmas.82384


Web Style

Mondal P, Mukherjee P, Das D, Mandal TK, Haldar P, Chatterjee SS, Dan U, Roy R. Rationale of using SARS-CoV-2 Rapid Antigen Test as a Point of care device in resource poor clinical set up: A comparative study in a peripheral health set up to meet up pandemic needs. https://jmas.in/?mno=82384 [Access: September 12, 2024]. doi:10.5455/jmas.82384


AMA (American Medical Association) Style

Mondal P, Mukherjee P, Das D, Mandal TK, Haldar P, Chatterjee SS, Dan U, Roy R. Rationale of using SARS-CoV-2 Rapid Antigen Test as a Point of care device in resource poor clinical set up: A comparative study in a peripheral health set up to meet up pandemic needs. J Med Allied Sci. 2022; 12(2): 47-51. doi:10.5455/jmas.82384



Vancouver/ICMJE Style

Mondal P, Mukherjee P, Das D, Mandal TK, Haldar P, Chatterjee SS, Dan U, Roy R. Rationale of using SARS-CoV-2 Rapid Antigen Test as a Point of care device in resource poor clinical set up: A comparative study in a peripheral health set up to meet up pandemic needs. J Med Allied Sci. (2022), [cited September 12, 2024]; 12(2): 47-51. doi:10.5455/jmas.82384



Harvard Style

Mondal, P., Mukherjee, . P., Das, . D., Mandal, . T. K., Haldar, . P., Chatterjee, . S. S., Dan, . U. & Roy, . R. (2022) Rationale of using SARS-CoV-2 Rapid Antigen Test as a Point of care device in resource poor clinical set up: A comparative study in a peripheral health set up to meet up pandemic needs. J Med Allied Sci, 12 (2), 47-51. doi:10.5455/jmas.82384



Turabian Style

Mondal, Protik, Purba Mukherjee, Debasis Das, Tapan Kumar Mandal, Piyali Haldar, Shiv Sekhar Chatterjee, Utpal Dan, and Ramaprasasd Roy. 2022. Rationale of using SARS-CoV-2 Rapid Antigen Test as a Point of care device in resource poor clinical set up: A comparative study in a peripheral health set up to meet up pandemic needs. Journal of Medical and Allied Sciences, 12 (2), 47-51. doi:10.5455/jmas.82384



Chicago Style

Mondal, Protik, Purba Mukherjee, Debasis Das, Tapan Kumar Mandal, Piyali Haldar, Shiv Sekhar Chatterjee, Utpal Dan, and Ramaprasasd Roy. "Rationale of using SARS-CoV-2 Rapid Antigen Test as a Point of care device in resource poor clinical set up: A comparative study in a peripheral health set up to meet up pandemic needs." Journal of Medical and Allied Sciences 12 (2022), 47-51. doi:10.5455/jmas.82384



MLA (The Modern Language Association) Style

Mondal, Protik, Purba Mukherjee, Debasis Das, Tapan Kumar Mandal, Piyali Haldar, Shiv Sekhar Chatterjee, Utpal Dan, and Ramaprasasd Roy. "Rationale of using SARS-CoV-2 Rapid Antigen Test as a Point of care device in resource poor clinical set up: A comparative study in a peripheral health set up to meet up pandemic needs." Journal of Medical and Allied Sciences 12.2 (2022), 47-51. Print. doi:10.5455/jmas.82384



APA (American Psychological Association) Style

Mondal, P., Mukherjee, . P., Das, . D., Mandal, . T. K., Haldar, . P., Chatterjee, . S. S., Dan, . U. & Roy, . R. (2022) Rationale of using SARS-CoV-2 Rapid Antigen Test as a Point of care device in resource poor clinical set up: A comparative study in a peripheral health set up to meet up pandemic needs. Journal of Medical and Allied Sciences, 12 (2), 47-51. doi:10.5455/jmas.82384