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Original Article
Online Published: 28 Jul 2020
 


Protective effects of hydroalcoholic extract of Nigella sativa seed against CCl4-induced blood oxidant / antioxidant changes and hepatorenal toxicity in rats

Ikenna Kingsley Uchendu, Ebuka Bitrus Nnedu, Henshaw Uchechi Okoroiwu, Ifeoma Blessing Ekeigwe.


Abstract
The liver and the kidneys are the main target organs of many harmful chemicals, toxins and some by-products of metabolic detoxification. Seeds with medicinal properties have been widely investigated for their therapeutic potentials against toxins, using animal models. We evaluated the protective effects of hydroalcoholic extract of Nigella sativa (HANS) against carbon tetrachloride (CCl4)-induced oxidative stress and hepatorenal toxicity in albino rats. Twenty five (25) albino rats, with average weight (105±5g), were randomly grouped into five groups: A-E, of five (5) rats per group. Group A rats served as normal control, Group B (Negative control) received intraperitoneal administration of CCl₄ (0.4ml/kg, i.p.) only, Group C received CCl₄ and low dose HANS (400mg/kg, oral), Group D received CCl₄ and high dose HANS (800mg/kg, oral), and Group E (Positive control), received CCl₄ and Vitamin C (200mg/kg, oral), for 7 days. The oxidative state of the blood and the hepatorenal toxicity were assessed by measuring serum levels of malodialdehyde (MDA), catalase (CAT), reduced glutathione (GSH), superoxide dismutase (SOD); total bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP); urea, creatinine, potassium (K+) and sodium (Na+) using standard methods. Histopathological analysis of the liver and kidney tissues was also carried out. Results revealed that HANS significantly stabilized blood oxidant/antioxidant imbalance, stabilized biochemical markers of hepatorenal injury, and preserved the histoarchitecture of the liver and kidney. The protective effect was dose-dependent, with low dose HANS (400mg/kg), showing better protection than the high dose HANS (800mg/kg). Hydroalcoholic extracts of Nigella sativa has antioxidant and hepatorenal protective abilities.

Key words: Antioxidants, CCl4, Hepatotoxicity, Medicinal plants, Nephrotoxicity, Nigella sativa, Oxidative stress


 
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How to Cite this Article
Pubmed Style

Uchendu IK, Nnedu EB, Okoroiwu HU, Ekeigwe IB. Protective effects of hydroalcoholic extract of Nigella sativa seed against CCl4-induced blood oxidant / antioxidant changes and hepatorenal toxicity in rats. J Med Allied Sci. 2020; 10(2): 52-61. doi:10.5455/jmas.83615

Web Style

Uchendu IK, Nnedu EB, Okoroiwu HU, Ekeigwe IB. Protective effects of hydroalcoholic extract of Nigella sativa seed against CCl4-induced blood oxidant / antioxidant changes and hepatorenal toxicity in rats. https://jmas.in/?mno=83615 [Access: September 12, 2024]. doi:10.5455/jmas.83615

AMA (American Medical Association) Style

Uchendu IK, Nnedu EB, Okoroiwu HU, Ekeigwe IB. Protective effects of hydroalcoholic extract of Nigella sativa seed against CCl4-induced blood oxidant / antioxidant changes and hepatorenal toxicity in rats. J Med Allied Sci. 2020; 10(2): 52-61. doi:10.5455/jmas.83615


Vancouver/ICMJE Style

Uchendu IK, Nnedu EB, Okoroiwu HU, Ekeigwe IB. Protective effects of hydroalcoholic extract of Nigella sativa seed against CCl4-induced blood oxidant / antioxidant changes and hepatorenal toxicity in rats. J Med Allied Sci. (2020), [cited September 12, 2024]; 10(2): 52-61. doi:10.5455/jmas.83615


Harvard Style

Uchendu, I. K., Nnedu, . E. B., Okoroiwu, . H. U. & Ekeigwe, . I. B. (2020) Protective effects of hydroalcoholic extract of Nigella sativa seed against CCl4-induced blood oxidant / antioxidant changes and hepatorenal toxicity in rats. J Med Allied Sci, 10 (2), 52-61. doi:10.5455/jmas.83615


Turabian Style

Uchendu, Ikenna Kingsley, Ebuka Bitrus Nnedu, Henshaw Uchechi Okoroiwu, and Ifeoma Blessing Ekeigwe. 2020. Protective effects of hydroalcoholic extract of Nigella sativa seed against CCl4-induced blood oxidant / antioxidant changes and hepatorenal toxicity in rats. Journal of Medical and Allied Sciences, 10 (2), 52-61. doi:10.5455/jmas.83615


Chicago Style

Uchendu, Ikenna Kingsley, Ebuka Bitrus Nnedu, Henshaw Uchechi Okoroiwu, and Ifeoma Blessing Ekeigwe. "Protective effects of hydroalcoholic extract of Nigella sativa seed against CCl4-induced blood oxidant / antioxidant changes and hepatorenal toxicity in rats." Journal of Medical and Allied Sciences 10 (2020), 52-61. doi:10.5455/jmas.83615


MLA (The Modern Language Association) Style

Uchendu, Ikenna Kingsley, Ebuka Bitrus Nnedu, Henshaw Uchechi Okoroiwu, and Ifeoma Blessing Ekeigwe. "Protective effects of hydroalcoholic extract of Nigella sativa seed against CCl4-induced blood oxidant / antioxidant changes and hepatorenal toxicity in rats." Journal of Medical and Allied Sciences 10.2 (2020), 52-61. Print. doi:10.5455/jmas.83615


APA (American Psychological Association) Style

Uchendu, I. K., Nnedu, . E. B., Okoroiwu, . H. U. & Ekeigwe, . I. B. (2020) Protective effects of hydroalcoholic extract of Nigella sativa seed against CCl4-induced blood oxidant / antioxidant changes and hepatorenal toxicity in rats. Journal of Medical and Allied Sciences, 10 (2), 52-61. doi:10.5455/jmas.83615


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